Optical Imaging Core

Overview of Services

The OIC offers imaging access to six microscopy systems: (1) a Leica SP8 3X white light inverted laser scanning confocal microscope, (2) a Nikon AXR inverted laser scanning confocal microscope, (3) a Nikon spinning disk laser inverted confocal microscope, (4) Nikon A1R inverted laser scanning confocal microscope, (5) Bruker Ultima two photon and (6) 3i lattice light sheet sheet microscope. Imaging techniques available through the core now include: high resolution microscopy, long-term live cell imaging, two-photon/intravital microscopy, spinning disk confocal imaging, deconvolution, fluorescence spectral detection,FRAP, calcium imaging, and immuno fluorescence. Investigators can maintain and grow live cells in a culture room equipped with biosafety cabinets, hypoxia and CO2 incubators (Room 2081).

Covid-19 Protocols

CLEANING EXPECTATIONS

• Wash and sanitize hands after entering equipment rooms and again before leaving.
• Soap and paper towels will be provided in 2073/2081.
• Prior to use please wipe down all washable surfaces with 70% ethanol soaked Kimwipes – prevent aerosolizing settled virus.
• Do not spray directly onto the microscopes.
• Clean all surfaces touched again at the end of your session.
• Pay special attention to eyepieces and the front body of the scopes that is being breathed on.
• We discourage the use of gloves to prevent a false sense of security.
• Clean all door handles inside and out prior to leaving the area.

SOCIAL DISTANCING EXPECTATIONS

• Users are asked to flip door occupancy signs whenever entering or leaving equipment rooms.
• Please share your contact information in reservation comments box for other users to reach out during transition if needed.
• Only one user is permitted per microscope room.
• Consider entering a space vacated by the previous user at least 15 minutes after the beginning of their own reservation.
• By delaying entering a room, a user can lower his/her peronal risk of exposure by allowing potential aerosolized virus to settle or clear through air exchange.

Leadership

Lance Rodenkirch | Managing Director | 608.263.3248 | larodenk@wisc.edu

Owen Tamplin Faculty Director

Location and hours of operation

Links and Resources

1. Optical Imaging Core Website

Contacts